I took the test the other day and I have listed questions and topics I remember below:
1. Create a 40% solution in 200mL of a compound with a 19:1 molecular weight ratio (76g, 4g)
2. Microsatellite instability and mismatch repair
3. What kind of bond histones have with DNA (ionic)
4. What amino acids compose histones (lysine and arginine)
5. Lymphoma mutations
6. Leukemia mutations
7. Forward and reverse primer
8. Factor V leiden (gave symptoms, answers were the mutation)
9. Calculate original concentration of 50X diluted RNA given A260 OD value.
10. Leading strand polymerase: delta
11. DNA repair polymerase: beta
12. Pyrosequencing and luciferase
13. Molecular beacon design (R-sequence-Q)
14. Which method uses visible light (pyrosequencing)
15. In NGS what is coverage referring to (# of times a base is read)
16. Number of SNPs in humans (10^7) [books says 5 million]
17. Know everything about PCR troubleshooting
18. FLT1
19. RNA storage condition for 6 months (DEPC water vs. EtOH)
20. Getting a twin with identifcal twin organ transplant
21. How long to keep obsolete document in lab according to CAP checklist
22. EtBr – neurotoxicity?
23. HIV viral load difference (0.5 log 10)
24. Which electropherogram is most informative (question show donor and recipient electropherograms and you have to choose which is the most informative)
25. Antibiotic resistance mechanism in MRSA (altered drug target, PBP2a?)
26. Irinotecan and UGT1A1 (what is the effect? Decrease drug metabolism)
27. What part of rna increases half-life and stability? (g cap)
28. Formamide decreases Tm by altering H bonding
29. Common form of DNA (right handed, B form)
30. Order to add to a pcr reaction (in forums it will say master mix, positive control, patient control, negative control but that is not an answer option. The answer I selected was master mix, patient sample, positive control, negative control)
31. Primer dimers are formed due to 3’ complementarity
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