Well, I took the test last week and did not pass (but I really didn't give myself enough time to study effectively). However, the test has changed and below is what I could remember:
•DNA and RNA pols
•Pyrosequencing (EX: Where does the signal come from?)
•CF type and specific mutation, carriers vs those that exhibit symptoms
•Hodgkens, Sickle-cell anemia,CF
•NGS (EX: Data type (file type FASTA), Databases available)
•Calculations (EX: PPV, V1C1=V2C2, A260 of 1 = 50µg/mL
•Hybridization
•EBV is a member of herpes
•Lymphomas
•IQCP (Ex:Risk)
•Sensitivity, Accuracy, Precision based off of real world examples
•CLIA Competency Testing/CLIA Proficiency Testing
•Read qPCR data
•Sequencing – Read
•If you have a “weak PCR product”, what to do to increase the yield?
Options: change annealing time, annealing temp, extension temp, decrease dNTPs
•What might interfere with a signal (using a quencher), primers with 3’ or 5’ Gs, ATs?
•Chlamydia detection
•Primer Design questions/Primer troubleshooting questions
•HIV viral load is 50 copies one week, and the next week 100,000. Doctor calls you with concern. What do you tell the doctor? That this is expected? That you need to re-test? That the drastic change is due to inhibitors? So on.
•A negative sample with some known positive shows what? Precision? Sensitivity? ….
•The electricity goes out but your thermocycler has a USP. However, the program had been interrupted during the outage. What do you do? Report and start over (new samples)? Report and calculate what cycle you are on? Just restart up the program? So on…
•The sample and a sample with a known internal positive both have problems. What do you do?
Restart from original RNA? Get new sample? So on…
•A primer of a reverse transcriptase reaction. How would you design? Over introns or exons? Over poly (t) tail? Genomic?
•Comparing a sample against a calibration curve shows, Precision? Accuracy? Specificity? Reproducibility?
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